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2090 次 Isolation and culture of smooth muscle cell 2017-3-16
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. 2.Kidneys and thoracic aorta were removed, rinsed with sterile ice-cold phosphate-buffer
2223 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr
2271 次 Trabecular cell monolayer culture 2011-9-2
Trabecular cell monolayer cultureOriginally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
2301 次 Isolation and culture of rat coronary microvascular endothelial cells 2011-5-11
Isolation and culture of rat coronary microvascular endothelial cells (CMVE)CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.Hearts mounted o
2263 次 Isolation and Culture of Human Liver Stem Cells 2011-4-1
Isolation and Culture of Human Liver Stem CellsMaterials and Methods1.Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy li