羅氏將推出多樣本混合捕獲實(shí)驗(yàn)方案優(yōu)化序列捕獲技術(shù)
羅氏NimbleGen將推出多樣本混合捕獲實(shí)驗(yàn)方案以及全基因組外顯子液相捕獲三代產(chǎn)品
二代測(cè)序技術(shù)正在不斷突破高通量以及低測(cè)序成本的極限。定向測(cè)序更是當(dāng)前最有效控制測(cè)序成本同時(shí)獲得關(guān)鍵序列信息的首選。NimbleGen即將推出序列捕獲前的多樣本混合實(shí)驗(yàn)方案，希望以此更好地優(yōu)化序列捕獲技術(shù)以配合二代測(cè)序平臺(tái)的高通量，進(jìn)而減少實(shí)驗(yàn)時(shí)間并降低測(cè)序費(fèi)用。這一新技術(shù)利用不同條形碼序列來(lái)結(jié)合不同樣本，然后混合一次實(shí)驗(yàn)中進(jìn)外顯子或定制目標(biāo)區(qū)域的液相捕獲。
羅氏NimbleGen的首席執(zhí)行官Frank Pitzer說(shuō)：“我們很高興向所有研究人員突出這個(gè)高效而且低成本的實(shí)驗(yàn)方案。相信通過(guò)這一方法，研究人員可以提高研究項(xiàng)目的樣本通量，以此增強(qiáng)研究項(xiàng)目在統(tǒng)計(jì)學(xué)上的重要性。”
多樣本混合實(shí)驗(yàn)方案的同時(shí)，新一代的外顯子液相捕獲產(chǎn)品也會(huì)同時(shí)推出。這一新產(chǎn)品將可捕獲64M的基因組序列，包括所有外顯子以及miRNA，它含與其他NimbleGen液相捕獲產(chǎn)品相同的2.1M高密度探針，以確保高效、均一、特異、全面的定向捕獲，將成為市場(chǎng)覆蓋面最廣的序列捕獲產(chǎn)品之一。
Pitzer先生介紹說(shuō)：“這個(gè)新的產(chǎn)品， NimbleGen SeqCap EX Exome Library v3.0延續(xù)了NimbleGen一貫以來(lái)產(chǎn)品的高效和均一的特點(diǎn)，這一點(diǎn)得到了行業(yè)內(nèi)的認(rèn)可，許多文獻(xiàn)中的實(shí)驗(yàn)結(jié)果也可以證明。例如在最近Nature Biotechonlogy雜志中刊登的一篇文章1，對(duì)于三種外顯子組捕獲產(chǎn)品的捕獲序列進(jìn)行測(cè)序后比較，在同樣獲得80M測(cè)序數(shù)據(jù)的情況下，NimbleGen有97%的目標(biāo)序列達(dá)到10x以上 的測(cè)序深度，而其他產(chǎn)品只有90%。此外，NimbleGen SeqCap EX Exome Library v3.0產(chǎn)品將覆蓋更廣泛的區(qū)域，包括RefSeq, CCDS Vega以及Ensemble Database中的外顯子相關(guān)區(qū)域�！�
于此同時(shí)，研究人員仍然可以選擇NimbleGen SeqCap EX Exome Library v2.0產(chǎn)品，它仍將是針對(duì)RefSeq數(shù)據(jù)庫(kù)的外顯子序列最為經(jīng)濟(jì)有效的測(cè)序捕獲工具。而兩項(xiàng)新產(chǎn)品的相關(guān)數(shù)據(jù)信息，將在最近在加拿大蒙特利爾舉辦的美國(guó)人類基因?qū)W年會(huì)中發(fā)布，敬請(qǐng)留意后續(xù)報(bào)道。
更多有關(guān)羅氏NimbleGen產(chǎn)品，請(qǐng)?jiān)L問(wèn)www.nimblegen.com.
文中所涉及的文獻(xiàn)
(1) Clark et al., Performance comparison of exome DNA sequencing technologies (2011)
Nature Biotechnology Published online 25 September 2011 doi:1038/nbt.1975
Roche NimbleGen Announces New Pre-capture Multiplexing for Target Enrichment Technology in Sequencing
With the decreasing cost and increasing throughput of sequencing, researchers require a high-performance, cost-effective sample preparation pipeline for targeted sequencing. To enable researchers to more readily match targeted sequencing sample preparation throughput to the ever increasing throughput of next-generation sequencing, Roche NimbleGen (SIX: RO, ROG; OTCQX: RHHBY) announces the imminent launch of a pre-capture multiplex target enrichment protocol. This new pre-capture multiplex protocol enables multiple DNA samples to be barcoded and captured in a single SeqCap EZ Library reaction for exome or custom capture experiments.
“We are extremely excited to provide researchers with a high performance, cost-effective pre-capture multiplex protocol that should allow researchers to increase the size of their studies, and thus, the statistical relevance,” stated Frank Pitzer, CEO of Roche NimbleGen.
The pre-capture multiplex protocolwill be launched for an additional, more comprehensive Exome capture product. This new product will employ the same high-density probe technology that ensures high capture efficiency in all of its existing SeqCap EZ products. However, the new Exome product will target 64Mb of coding exons and miRNAs, providing researchers with an efficient target enrichment product with the most comprehensive coverage of coding regions.
“The new extension of our target enrichment portfolio, NimbleGen SeqCap EZ Exome Library v3.0, will provide researchers with the same industry-renown performance and uniformity that researchers worldwide have proven in numerous recent publications. In one recent study in Nature Biotechnology1, with 80M reads, ~97% of the target bases are covered by more than 10-fold using NimbleGen SeqCap EZ where only ~90% of the target bases are covered by competitive technologies. Additionally, SeqCap EZ Exome Library v3.0 will target the most comprehensive collection of exons in the market as defined by the RefSeq, CCDS, Vega, and Ensembl databases,” Pitzer noted. Roche NimbleGen will continue to offer the high-performance SeqCap EZ Exome v2.0 product, as an efficient tool for researchers who want to generate extremely cost-effective sequencing data for RefSeq exons.
Roche plans to release further information of both the pre-capture multiplexing protocol and the NimbleGen SeqCap EZ Exome v3.0 at the American Society of Human Genetics (ASHG) annual meeting (for more information visit Roche at ASHG booth number 502) next week in Montreal, Canada.
For more information about Roche NimbleGen, please visit www.nimblegen.com
(1) Clark et al., Performance comparison of exome DNA sequencing technologies (2011)
Nature Biotechnology Published online 25 September 2011 doi:1038/nbt.1975
- 潔特生物推出新品ProGro™胎牛血清，可免費(fèi)試用
- 昊量時(shí)間相關(guān)單光子計(jì)數(shù)器Time Tagger v2.17發(fā)布
- 10x Genomics全新入門級(jí)單細(xì)胞系統(tǒng)Chromium Xo上市
- 活動(dòng)回顧：奧豪斯新品上市北京站圓滿落幕
- 東勝龍 ETC811X 基因擴(kuò)增儀重磅上市
- 譜鐳光電推出結(jié)構(gòu)緊湊型飛秒脈沖測(cè)量?jī)xFROG
- 推進(jìn)數(shù)智化，Eppendorf 新品96通道智能移液系統(tǒng)問(wèn)世
- 博大博聚動(dòng)態(tài)細(xì)胞成像儀上市，助力細(xì)胞分析自動(dòng)化
- 昊量光電代理商—法國(guó)ALPAO新品變形鏡DM57-15上線
- 新品：Pribolab®光化學(xué)柱后衍生器MDU
- 基因公司推出新品—TEROS 22土壤水勢(shì)/溫度傳感器
- 中喬新舟推出新品自研周細(xì)胞完全培養(yǎng)基
- 新品：艾普拜精衛(wèi)自動(dòng)PCR擴(kuò)增儀震撼來(lái)襲
- 玉研新品：動(dòng)物實(shí)驗(yàn)縫合針線震撼發(fā)布
- 奧豪斯隆重推出新品Guardian 5000™加熱攪拌器
- 10x Genomics全新入門級(jí)單細(xì)胞系統(tǒng)Chromium Xo上市
- 首期“單細(xì)胞空間組學(xué)應(yīng)用科學(xué)沙龍”圓滿結(jié)束
- 安捷倫啟用生物制藥伴隨診斷服務(wù)實(shí)驗(yàn)室
- Illumina研究獎(jiǎng)邀您贏取1008個(gè)樣本的甲基化檢測(cè)機(jī)會(huì)
- 真邁GenoLab M Dx測(cè)序儀獲批NMPA三類醫(yī)療器械注冊(cè)證
- 10x邀您觀看2024空間組學(xué)高峰論壇線上同步直播
- 10x Genomics邀您參加2024中國(guó)空間組學(xué)高峰論壇
- 因美納擴(kuò)展全景變異分析應(yīng)用，賦能NovaSeq X用戶
- 因美納宣布全新公司戰(zhàn)略，加速價(jià)值創(chuàng)造
- 森西賽智參展回顧：第五屆全國(guó)腫瘤細(xì)胞生物學(xué)年會(huì)
- 生物育種基因工程與基因發(fā)掘利用技術(shù)研討會(huì)通知
- 講座：?jiǎn)渭?xì)胞多組學(xué)測(cè)序在植物非生物脅迫中的應(yīng)用
- INTEGRA攜手Parse實(shí)現(xiàn)scRNA-seq測(cè)序工作流程自動(dòng)化
- 10x Genomics同行引薦計(jì)劃:免費(fèi)試劑或儀器保修優(yōu)惠
- 2024中國(guó)國(guó)際輔助生殖大會(huì)通知
Copyright(C) 1998-2024 生物器材網(wǎng) 電話：021-64166852;13621656896 E-mail：info@bio-equip.com