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Stable Cell Line Services

Stable Cell Line Services


Stable Cell Line Services
Stable Cell Line Services
服務(wù)類別:其他生物研發(fā)總訪問:2029
最后更新:2012-4-23半年訪問:23
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服務(wù)商: 北京百普賽斯生物科技有限公司 查看該公司所有服務(wù) >>
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Development of a stable cell line based on CHO or NS0 for production of recombinant proteins and monoclonal antibodies is unavoidable for some applications such as clinical use, when mammalian cell expression system is pursued. However, stable cell line generation is mostly thought as time and cost-consuming work.

ACRObiosystems' provide commercial and research CHO(DG44, DUKX-B11, CHO-S or CHO-K1) cell line based on its proprietary expression vector, high throughput cell screening technologies and serum,animal components free process without any adaptation issue in a significantly reduced timeline

Service Specification


Service Content Approaches  Deliverables Timeline

Gene synthesizing
&Plasmid construction

  • In house developed codon optimization
  • Gene synthesizing and verification(in house)
  • Clone to proprietary mammalian expression vector
  • Gene sequencing report
  • Constructed plasmid
25days
Transient expression evaluation
  • Shake flaks scale transient transfection
  • Elisa based expression level measurement 
  • Expression feasibility
10days
Transfection and clone screening
  • 3 approaches, 3 rounds of clone selection
  • 5 top clones expand to shake flask scale for evaluation
  • 50 PDL stability evaluation upon request(additional time needed)
  • Three top clones
  • Expression stability data
  • Sterility/mycomplasma test
  • Other QC test upon request
40-60days
Process Development(optional)
  • Mutiple in-house developed chemically defined media/feeds platform screening and optimization
  • High throughput purification processes screening via DoE
  • Analytical assay development
  • Milligram to gram purified proteins
  • Process report
Quote
Production Service(optional)
  • 10L-50L bioreactor run
  • Chemically defined medium/feeds
  • Process control
  • Purified proteins
  • Process profile
  • Production report and other documentation upon request
Quote

Quotations and Ordering

You can place the order on line and make the payment with credit card or Paypal. Also you can place the order off-line by phone, email, fax with a formal PO (Purchase Order) or credit card. We recommend that you submit your service requests and orders on line. 

For questions and quotation requests, please submit your on-line request. Our customer specialist will get back to you in 24 hours from Monday through Friday. However, you can also contact us by email, phone, or fax.

Email:
Phone: +1 301-825-5518(US)   +44 20-8123-6518(EU)   +86 400-685-2052(AP)
Fax: +1 301-500-2214

 

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