微電極陣列（Med-64）對(duì)野生型(WT)和W/W（v）型小鼠腸起搏器活動(dòng)的研究

Cajal間質(zhì)細(xì)胞中的肌間神經(jīng)叢區(qū)域（ICC-MyP）能形成一個(gè)網(wǎng)絡(luò)，并產(chǎn)生基底起搏電活動(dòng)。這種形態(tài)特征使我們相信，這些細(xì)胞對(duì)胃腸道協(xié)調(diào)運(yùn)動(dòng)是非常重要的。我們希望找出一種新的方法來(lái)對(duì)ICC-MyP的電場(chǎng)活動(dòng)和網(wǎng)絡(luò)功能作出評(píng)估。約1毫米面積的ICC-MyP 區(qū)域的場(chǎng)電位通過(guò)極距為150um的8x8微電極陣列進(jìn)行測(cè)量, 含硝苯吡啶和河豚毒素的細(xì)胞外液能抑制平滑肌細(xì)胞和神經(jīng)元。我們也比較了野生型（WT）和W/W（v）型小鼠回腸肌肉空間電場(chǎng)活動(dòng)。通過(guò)3維時(shí)空分析法分析表明，WT型小鼠的基底電活動(dòng)與傳播延遲是同步進(jìn)行的，而在W/W（v）型小鼠中同樣信號(hào)幅度卻較小和出現(xiàn)規(guī)律也沒(méi)有那么正常的現(xiàn)象。WT型小鼠的功率譜中有一個(gè)突出的峰值，并與ICC-MyP的起搏器的活動(dòng)頻率對(duì)應(yīng)，而W/W（v）型中是沒(méi)有這個(gè)峰值的。因此，在9.4-27.0每千次的光譜功率中，WT野生型遠(yuǎn)遠(yuǎn)超過(guò)了W/W（v）型 。最后，微電極陣列測(cè)量表明，該網(wǎng)絡(luò)不僅能產(chǎn)生，而且還調(diào)節(jié)基底電活動(dòng)。由ICC網(wǎng)絡(luò)形態(tài)和功能損傷所引起的胃腸運(yùn)動(dòng)失調(diào)將會(huì)在以后更加深入的研究中被發(fā)現(xiàn)。

Microelectrode array evaluation of gut pacemaker activity in wild-type and W/W(v) mice.

Nakayama S, Ohishi R, Sawamura K, Watanabe K, Hirose K.

Department of Cell Physiology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. h44673a@nucc.cc.nagoya-u.ac.jp

Interstitial cells of Cajal in the myenteric plexus region (ICC-MyP) form a network and generate basal pacemaking electrical activity. This morphological feature leads us to believe that these cells may be essential for the coordinating actions of gastrointestinal (GI) motility. We aim to propose a new method for functional assessment of ICC electrical activity and its network. Field potentials in a approximately 1 mm(2) region were simultaneously measured using an 8x8 microelectrode array (MEA) with a polar distance of 150 microm. The extracellular solution contained nifedipine and tetrodotoxin (TTX) to suppress activities of smooth muscle cells and neurons, respectively. We compared spatial electrical activities between ileal muscle preparations from wild-type (WT) and W/W(v) mice. In spatio-temporal analyses, basal electrical activities were well synchronized with a propagation delay in WT, while those in W/W(v) were small in amplitude and irregular in occurrence. The power spectrum in WT had a prominent peak corresponding to the frequency of ICC-MyP pacemaker activity, while that of W/W(v) lacked it. Consequently, the ratio of the spectral power in 9.4-27.0 cpm was significantly larger in WT than in W/W(v). In conclusion, MEA measurements demonstrated that the network-forming ICC-MyP not only generates but also coordinates basal electrical activities. Disorders of GI motility based on morphological and functional impairments of ICC network with the range of several hundreds of micrometers, could be uncovered in future extensive studies.

文章來(lái)源：
http://www.ncbi.nlm.nih.gov/pubmed/19576758?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_
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