氧分子流作為黑頭呆魚胚胎的生理應激指標：一個實時水質(zhì)生物監(jiān)測系統(tǒng)

關鍵詞：非損傷微測技術(NMT)，環(huán)境監(jiān)測，氧氣(Oxygen flux)，水質(zhì)

參考文獻：Sanchez BC, et al. Environ. Sci. Technol , 2008, 42, 7010-7017

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Abstract:

The detection of harmful chemicals and biological agents in real time is a critical need for protecting freshwater ecosystems. We studied the real-time effects of five environmental contaminants with differing modes of action (atrazine, cadmium chloride, pentachlorophenol, malathion, and potassium cyanide) on respiratory oxygen consumption in 2-day postfertilization fathead minnow (Pimephales promelas) eggs. Our objective was to assess the sensitivity of fathead minnow eggs using the self-referencing micro-optrode technique to detect instantaneous changes in oxygen consumption after brief exposures to low concentrations of contaminants. Oxygen consumption data indicated that the technique is indeed sensitive enough to reliably detect physiological alterations induced by four of the five contaminants. After 2 h of exposure, we identified significant increases in oxygen consumption upon exposure to pentachlorophenol (100 and 1000 μg/L), cadmium chloride (0.0002 and 0.002 μg/L), and atrazine (150 μg/L). In contrast, we observed a significant decrease in oxygen flux after exposures to potassium cyanide (44and66μg/L)andatrazine (1500 μg/L). No effects were detected after exposures to malathion (200 and 340 μg/L). Our work is the first step in development of a new technique for physiologically coupled biomonitoring as a sensitive and reliable tool for the detection of environmental toxicants.