在培養(yǎng)脊髓運(yùn)動(dòng)神經(jīng)元過程中所形成由神經(jīng)元和網(wǎng)絡(luò)活動(dòng)構(gòu)成的網(wǎng)絡(luò)

這是第一次報(bào)告多電極記錄運(yùn)動(dòng)神經(jīng)元網(wǎng)絡(luò)。從E15大鼠脊髓腹角分離出來的神經(jīng)細(xì)胞放在Med-64探針里培養(yǎng)。大多數(shù)培養(yǎng)的神經(jīng)元具有神經(jīng)絲、膽堿乙酰轉(zhuǎn)移酶和Hb9 等運(yùn)動(dòng)神經(jīng)元的特點(diǎn)。運(yùn)動(dòng)神經(jīng)元網(wǎng)絡(luò)的活動(dòng)特點(diǎn)是具有單個(gè)細(xì)胞尖峰，并且是自發(fā)的。尖峰和網(wǎng)絡(luò)突發(fā)是由γ-氨基丁酸拮抗劑 (GABA)和乙酰膽堿刺激引起的，也能被GABA和谷氨酸受體拮抗劑本身所抑制。培養(yǎng)的胚胎運(yùn)動(dòng)神經(jīng)元網(wǎng)絡(luò)是一個(gè)研究運(yùn)動(dòng)神經(jīng)元發(fā)展、運(yùn)動(dòng)神經(jīng)疾病生理及病理機(jī)制的理想模型系統(tǒng)。

Neuronal and network activity in networks of cultured spinal motor neurons.

Zhang HM, Robinson N, Gómez-Curet I, Wang W, Harrington MA.

Department of Biological Sciences, Delaware State University, Dover, DE 19901, USA.

This is the first report of multielectrode recordings from networks of cultured motor neurons. Neurons isolated from the ventral horns of spinal cords of E15 rats were cultured on MED64 probes. The majority of the neurons in the cultures are positive for neurofilament, choline acetyltransferase, and Hb9, characteristics of motor neurons. The activity of the motor neuron network is characterized by spiking of individual cells as well as spontaneous, synchronized bursts involving all active electrodes. Both spiking and network bursts are stimulated by GABA antagonists and acetylcholine, and are inhibited by GABA itself and glutamate antagonists. Networks of cultured embryonic motor neurons make a good model system for studying motor neuron development and physiology as well as the pathophysiology of motor neuron disease.

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