Oncogene文章：microRNA-133b是宮頸癌發(fā)生與發(fā)展的關(guān)鍵因素

Oncogene文章：microRNA-133b是宮頸癌發(fā)生與發(fā)展的關(guān)鍵因素
 

　　宮頸癌（cervical carcinoma）是女性第二大惡性腫瘤，并且在年輕女性中發(fā)病率高。2002年全世界的新發(fā)病例為50萬人，死亡病例為27萬人。根據(jù)中國疾病預(yù)防與控制中心公布的數(shù)據(jù)，我國每年宮頸癌新發(fā)病例為13.15 萬人，約占世界總數(shù)的26%，死亡病例為5.3 萬人，約占世界總數(shù)的20%。因此，我國的宮頸癌防治形勢非常嚴(yán)峻。
 
　　在99.8%的宮頸癌患者中均可檢測到HPV感染。但單獨(dú)的HPV 感染不足以引起宮頸癌，細(xì)胞本身必須發(fā)生相應(yīng)的變化，才能最終引起宮頸癌。清華大學(xué)的程京教授與新疆醫(yī)科大學(xué)第一附屬醫(yī)院的溫浩教授合作，利用生物芯片平臺研究了宮頸癌組織中microRNA的表達(dá)變化，發(fā)現(xiàn)了20種microRNA在宮頸癌的發(fā)生和發(fā)展過程中表達(dá)量發(fā)生了改變。其中microRNA-133b在從宮頸上皮內(nèi)瘤樣病變到侵潤癌的發(fā)展過程中，表達(dá)量逐步升高。功能學(xué)實驗結(jié)果表明，這一microRNA具有促進(jìn)宮頸癌發(fā)生和發(fā)展的作用。該microRNA有可能作為臨床早期診斷的新型宮頸癌標(biāo)志物。
　　上述研究成果已申請專利，研究論文已在Oncogene雜志網(wǎng)站在線發(fā)表。其中的博奧生物晶芯^®哺乳動物miRNA芯片服務(wù)與Real time RT-PCR服務(wù)在博奧生物有限公司完成。

原文摘要：

　　MicroRNA-133b is a key promoter of cervical carcinoma development through the activation of the ERK and AKT1 pathways
 
　　We report that elevated microRNA-133b (miR-133b) acts as an oncogene in human cervical carcinoma to promote tumorigenesis and metastasis. In situ hybridization confirmed that miR-133b is localized in proliferating human cervical carcinoma cells with levels progressively elevating throughout advancing stages. Cellular studies showed that miR-133b enhances cell proliferation and colony formation by targeting mammalian sterile 20-like kinase 2 (MST2), cell division control protein 42 homolog (CDC42) and ras homolog gene family member A (RHOA), which subsequently results in activation of the tumorigenic protein kinase B alpha (AKT1) and mitogenactivated protein kinase (ERK1 and ERK2, here abbreviated as ERK) signaling pathways. Mouse experiments revealed that upregulation of miR-133b in cervical carcinoma cells strongly promotes both in vivo tumorigenesis and independent metastasis to the mouse lung. The data indicates that upregulation of miR-133b shortens the latency of cervical carcinoma. Together, these findings suggest that miR-133b could be a potent marker for the early onset of cervical carcinoma.
 
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