eBioscience ELISA試劑盒使用手冊(cè)（二）

Q. 一步法Instant ELISA試劑盒，能做多少個(gè)檢測(cè)？
How many tests can I perform with an Instant ELISA Kit?

Instant ELISA包含128 tests。 其中包含可以做8個(gè)點(diǎn)復(fù)孔標(biāo)曲，共有32孔做標(biāo)曲，96孔檢測(cè)樣本。
With an Instant ELISA, 128 tests (includes samples plus standards) can be performed. The number of tests results from the following composition:
One 96 well plate designed only for samples; 96 samples
Two 8-point standard curve in duplicates; 32 wells; thus the kit can be used at two different time points, each with their own standard curve.

Q. 我需要將標(biāo)準(zhǔn)品加到酶標(biāo)孔中嗎？
Do I have to add recombinant standard to the wells?

不需要。標(biāo)準(zhǔn)品已經(jīng)凍干在酶標(biāo)孔中，不需要再另外加入。
No, the recombinant standard is included as a component of the lyophilized reagents in the wells.

Q. 酶標(biāo)孔中需要加二抗嗎？
Do I have to add the secondary antibody to the wells?

不需要。二抗也已經(jīng)被凍干在酶標(biāo)孔中。
No, the secondary antibody is included as a component of the lyophilized reagents in the wells.

Q. 樣本檢測(cè)之前，需要稀釋嗎？
Do I have to dilute my samples before adding them to the wells?

A. 一般情況下，使用Instant ELISA檢測(cè)，樣本不需要稀釋。但是具體請(qǐng)您參考隨貨說明書。In general, one can use undiluted samples with the Instant ELISA. Please refer to the product manual to determine if dilution of the sample is required for your kit of interest. The volume of sample required for the individual kits will also be listed in the product manual.

Q. 不同批次試劑盒，標(biāo)準(zhǔn)品的溶解體積一樣嗎？
Is the reconstitution volume of the standards the same for different lot numbers?

對(duì)大部分Instant ELISA 來說，標(biāo)準(zhǔn)品孔和空白孔的溶解體積是不一樣的，因?yàn)樗鼈兣�次特異性的。�?qǐng)您務(wù)必參考隨貨收到的產(chǎn)品說明書。
For some Instant ELISA kits, the reconstitution volume of the standard and blank wells may differ (these components are lot specific). Please use the manual provided in the Kit for specific volumes to use.

Q. 樣本濃度如何計(jì)算呢？
How do I calculate my samples?

樣本濃度稀釋因子請(qǐng)參考具體說明書。
The calculation factor is always stated in the manual.