如何利用脈沖場電泳系統(tǒng)進行長片段基因組DNA質(zhì)控？
瀏覽次數(shù)：823 發(fā)布日期：2023-9-28
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如何進行長片段基因組DNA質(zhì)控？—Pippin Pulse脈沖場電泳系統(tǒng)
隨著三代測序技術(shù)的發(fā)展和普及，全基因組測序技術(shù)也取得了長足發(fā)展�；蚪MDNA文庫的制備質(zhì)量直接影響測序結(jié)果，因而基因組DNA的質(zhì)控越來越受到重視。而實驗室常規(guī)的瓊脂糖凝膠電泳只能分離20kb以下的DNA分子，當(dāng)DNA分子大于20kb時，就無法實現(xiàn)有效分離。
針對超長DNA片段的分離和質(zhì)控，美國Sage Science公司擁有超高性價比的Pippin Pulse脈沖場電泳系統(tǒng)。Pippin Pulse使用簡單，無專用耗材，自帶預(yù)設(shè)程序，能很好的電泳分離0.5Kb到430Kb的基因組DNA片段。Pippin Pulse具有一個簡單的軟件界面，自帶6種預(yù)設(shè)程序，可以直接分離多種不同大小范圍DNA長片段，此外，軟件還支持自定義模式，用戶可以自編程分離不同范圍的DNA片段。Pippin Pulse包括電源、電泳槽、操控儀器的電腦和用戶手冊，無特殊耗材，使用成本低，經(jīng)濟耐用。
Pippin Pulse脈沖場電泳系統(tǒng)
Pippin Pulse脈沖場電泳系統(tǒng)預(yù)設(shè)程序
Pippin Pulse脈沖場電泳系統(tǒng)結(jié)果圖示
Pippin Pulse脈沖場電泳系統(tǒng)應(yīng)用案例（部分展示）
1. Pippin Pulse 與PacBio三代測序儀聯(lián)用，控制文庫片段分布，充分發(fā)揮長片段測序優(yōu)勢
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2. Pippin Pulse 與 Nanopore三代測序儀聯(lián)用，質(zhì)控更長、更高質(zhì)量的DNA，進一步提升測序長度
- Long, Meng, et al. "Comparative genomic analysis provides insights into taxonomy and temperature adaption of Aeromonas salmonicida." Journal of Fish Diseases 46.5 (2023): 545-561.
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- Belser, Caroline, et al. "Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps." Nature plants 4.11 (2018): 879-887.
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3. Pippin Pulse 與 Bionano分子光譜儀聯(lián)用，確保HMW DNA，保證分析結(jié)果準(zhǔn)確性
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- Canaguier A, Guilbaud R, Denis E, et al. Oxford Nanopore and Bionano Genomics technologies evaluation for plant structural variation detection[J]. BMC genomics, 2022, 23(1): 1-17.
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- Shukla H, Suryamohan K, Khan A, et al. Near-chromosomal de novo assembly of Bengal tiger genome reveals genetic hallmarks of apex-predation[J]. bioRxiv, 2022.
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- Rhie, Arang, et al. "Towards complete and error-free genome assemblies of all vertebrate species." Nature 592.7856 (2021): 737-746.
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- Belser, Caroline, et al. “Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing.” Communications biology 4.1 (2021): 1047.
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- Zheng, Yuting, et al. "Rapid prenatal diagnosis of Facioscapulohumeral Muscular Dystrophy 1 by combined Bionano optical mapping and karyomapping." Prenatal Diagnosis 40.3 (2020): 317-323.
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環(huán)亞生物科技有限公司作為美國Sage Science公司Pippin Pulse脈沖場電泳系統(tǒng)在中國區(qū)的獨家代理商，自2011年以來將Sage Science的產(chǎn)品線引入國內(nèi)，一直為國內(nèi)用戶提供專業(yè)的全自動核酸片段回收系統(tǒng)的安裝測試、應(yīng)用培訓(xùn)，技術(shù)支持與售后維護工作，贏得客戶的一致好評與信任。環(huán)亞生物科技有限公司將一如既往地支持越來越多的Sage Science用戶。
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