多因子生物標志物應(yīng)用于早期特異性診斷漿液性卵巢癌診斷

由于大多數(shù)患者是在晚期確診，卵巢癌仍然是最致命的婦科惡性腫瘤。沒有單一的生物標志物具有所需的靈敏度和特異性。

耶魯大學醫(yī)學院婦產(chǎn)科，哈佛大學布列根和婦女醫(yī)院（Brigham and Women’s Hospital，BWH），丹娜法伯癌癥研究院，約翰霍普金斯大學醫(yī)學院西德尼Kimmel綜合癌癥中心等，聯(lián)合開發(fā)了一組新的生物標志物，用于早期發(fā)現(xiàn)高級別漿液性卵巢癌。結(jié)果發(fā)表在最新一起婦產(chǎn)科腫瘤權(quán)威雜志：Gynecologic Oncology。 

臨床研究設(shè)計：

1. 候選因子：12個：IL-1Ra, IL-6, Dkk-1, uPA, E-CAD, ErbB2, SLPI, HE4, CA125, LCN2,MSLN, and OPN

2. 研究對象： 172病人（有婦科良性病變，或高級別漿液性卵巢腺癌等)

3. 技術(shù)平臺： Ella Simple Plex （ProteinSimple）

4. 結(jié)果：CA125, HE4, E-CAD, IL-6 四因子組成的panel具有特異性診斷漿液性卵巢癌的臨床價值。     

a)      卵巢癌病人versus無腫瘤對照組：特異性100%，陽性預(yù)測價值100%，靈敏度 86.4%

b)      卵巢癌病人versus婦科良性疾病對照組：特異性100%，陽性預(yù)測價值100%，靈敏度 87.2%

c)      卵巢癌病人versus無婦科良性疾病對照組：特異性100%，陽性預(yù)測價值100%，靈敏度 98.4%

d)      早期漿液性卵巢癌 Versus無腫瘤對照組：特異性95.7%，陽性預(yù)測價值88.9%，靈敏度 84.2%  

文中，作者特別注明選擇Ella simple plex進行本項研究的原因：

Our study is one such effort to identify a panel of multiple serum biomarkers which is not just sensitive and specific, but also time-efficient and cost-effective. Simple Plex™is a new multi-analyte immunoassay platform, which has been proven to have significant advantages over traditional approaches in terms of low volume requirements (2.5 to 25 μL), sensitivity, and reproducibility [34,35]. Simple Plex™ eliminates inconsistency in sample handling and  produces results in a short amount of time, thus providing results with minimal error and in timely fashion. Simplex Plex™ costs much less and would be more cost-efficient to analyze multiple biomarkers.

原文信息：

A novel multiple biomarker panel for the early detection of high-grade
serous ovarian carcinoma，C. Han et al. / Gynecologic Oncology xxx (2018) xxx–xxx，https://doi.org/10.1016/j.ygyno.2018.03.050.

作者單位：

1. Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA
2.  Department of Biostatistics, University of Arkansas for Medical Science, Little Rock, AR 72205, USA
3. Department of Cancer Biology, Department of Oncologic Pathology, Blais Proteomic Center, Dana-Farber Cancer Institute, Boston, MA 02115, USA
4. Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
5.Division of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
6.Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
7.Division of Gynecologic Oncology, Laura and Isaac Perlmutter Cancer Center, NYU Langone Health, New York, NY 10016, USA
8.Department of Obstetrics and Gynecology, Penn Ovarian Cancer Research Center, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA
9.Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT 06510, USA

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