wes微量樣品臨床生物標(biāo)志物定量檢測
典型的臨床樣本是稀缺的科學(xué)研究材料，臨床研究需要定量的研究工具，因而在臨床研究中針對微量樣本，實現(xiàn)定量檢測是技術(shù)平臺必不可少的要求。
最近歐洲最頂級的醫(yī)學(xué)研究中心薩拉曼卡大學(xué)醫(yī)學(xué)院癌癥研究中心，血液科的臨床科學(xué)家，在頂級血液病雜志haematologica發(fā)表方法學(xué)文章，A novel nano-immunoassay method for quantification of proteins from CD138 purified myeloma cells: biological and clinical utility。該文nano-immunoassay method為ProteinSimple Wes系統(tǒng)。
該研究選用63例多發(fā)性骨髓瘤，并分選其中CD138陽性細(xì)胞，提取蛋白質(zhì)及核酸用于后續(xù)檢測。
文章中，作者檢測了包括內(nèi)參蛋白GAPDH在內(nèi)的13種白質(zhì)
使用Wes系統(tǒng)建立絕對定量檢測模型
定量分析63例臨床樣本中13種白質(zhì)的表達(dá)水平
文中作者闡述了使用Wes nano-immunoassay method進(jìn)行此項臨床研究的原因：
1.The recent development of a method based on the combination of capillary nano-electrophoresis with immunoassay (CNIA), also known as Simple Western, requires only very small amounts of sample to be able to measure protein expression 3,7. This technical advance makes it possible to analyze the expression of 50-100 proteins in a single MM sample.
使用微量樣本，因而使得一份多發(fā)性骨髓瘤樣本可以進(jìn)行50-100個蛋白檢測成為可能。
2.Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation
準(zhǔn)確定量CD138分選細(xì)胞蛋白質(zhì)表達(dá)
3.This makes the CNIA platform a fast, effective and accurate tool for exploring the impact of different proteins on MM survival and for investigating new protein biomarkers that could help predict the response to new drugs that directly target specific proteins.
建立快速，搞笑，準(zhǔn)確的生物標(biāo)志物發(fā)現(xiàn)的方法，用于預(yù)測新藥的生物學(xué)反應(yīng)
