DYKDDDDK Fab-Trap™ Agarose是高效免疫沉淀(IP)Flag®標(biāo)記蛋白質(zhì)的高級(jí)親和樹(shù)脂。DYKDDDDK Fab-Trap™ Agarose由一個(gè)Fab片段組成,該片段與瓊脂糖珠共價(jià)結(jié)合。
應(yīng)用
Immunoprecipitation (IP) / Co-IP 免疫沉淀(IP)/共沉淀
Immunoprecipitation (IP) / Co-IP質(zhì)譜(MS)
Protein purification 蛋白質(zhì)純化
ChIP/ RIP analysis ChIP/RIP分析
On-bead enzyme assays 微珠酶分析
實(shí)驗(yàn)結(jié)果
DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.
訂貨信息
DYKDDDDK Immunoprecipitation Starter Pack
DYKDDDDK Fab-Trap™ Agarose (20 reactions, product code ffa-20) for immunoprecipitation, 3xDYKDDDDK-peptide (1mg, fp-1) for elution and DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20UL) for detection in Western blots.
Starter Pack (ffap) 包括以下組分:
· DYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20),
· 3xDYKDDDDK-peptide (1mg, fp-1) and
· DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20).
默瑞生物
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