Rab11-GTP 小鼠單抗實(shí)驗(yàn)使用及操作手冊(cè)

瀏覽次數(shù)：741　發(fā)布日期：2023-3-16　來源：費(fèi)斯德生物官網(wǎng)

Product Description

Pioneering GTPase and Oncogene Product Development since 2010

Rab11-GTP

Catalog Number: 26919

Gene Symbol: RAB11

Description: Anti-Rab11-GTP Mouse Monoclonal Antibody

Background: Small GTPases of the Rab-family are crucial elements of the machinery that controls

membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.

Immunogen: Recombinant full length protein of active

Rab11

Tested Applications: IP, IHC

Recommended Dilutions:

IP: 1 g for 1~2 mg total cellular proteins.

Immunoprecipitation and Western blot:

IHC: 1:50~100 dilution

Concentration: 1 mg/ml

Host: Mouse

Clonality: Monoclonal

Isotype: IgG2a, IgG2b P

Purity: Purified from ascites

Format: Liquid

Preservative: No

2+2+

Constituents: PBS (without Mg and Ca ), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti- Rab11-GTP antibody

recognizes active Rab11 from vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze /

thaw cycles

Rab11 Activation Assay.

Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPS (lane right). Immunoprecipitation

was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC APPLICATIONS

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