tRNA測(cè)序服務(wù)

tRNA測(cè)序服務(wù)    

    轉(zhuǎn)運(yùn)RNA（tRNA）是一類廣泛存在于生物體內(nèi)，在肽鏈合成中負(fù)責(zé)氨基酸轉(zhuǎn)運(yùn)的非編碼小RNA分子。最新研究表明，tRNA除參與肽鏈合成外，還參與多種生理病理學(xué)過程[1]。不同機(jī)體組織與細(xì)胞的轉(zhuǎn)錄組存在明顯差異，且不同mRNA的密碼子使用偏好性不同，使得所需的tRNA庫存在顯著差異。tRNA庫與轉(zhuǎn)錄組密碼子的匹配度影響著翻譯效率和準(zhǔn)確性[2-4]。增殖[5]、分化[5, 6]和凋亡[7]等細(xì)胞生理狀態(tài)都受到tRNA庫變化的影響。再者，許多研究表明tRNA庫在腫瘤[5, 8-12]、Ⅱ型糖尿病[13]、亨廷頓舞蹈癥[14]以及病毒感染[15]等疾病的發(fā)生發(fā)展中起著重要的調(diào)控作用。全長(zhǎng)tRNA分子具有非常穩(wěn)定的二級(jí)結(jié)構(gòu)和高水平的核苷酸修飾（tRNA功能發(fā)揮所必須的），這些都會(huì)嚴(yán)重影響高通量測(cè)序文庫構(gòu)建過程反轉(zhuǎn)錄酶的全長(zhǎng)延伸活性以及核酸序列的比對(duì)分析，從而導(dǎo)致常規(guī)small RNA建庫方案針對(duì)tRNA分子的文庫構(gòu)建失敗及分析結(jié)果的不準(zhǔn)確。

    康成生物在現(xiàn)有新型tRNA測(cè)序方案Hydro-tRNAseq (Cell Reports,2017)的基礎(chǔ)上進(jìn)行了進(jìn)一步的優(yōu)化，添加修飾核苷酸的去甲基化處理流程，更為優(yōu)勢(shì)地克服了tRNA測(cè)序的各個(gè)難點(diǎn)，高檢出率且準(zhǔn)確定量各類tRNA分子（圖1）。

  圖1: 康成生物tRNA測(cè)序流程圖

康成生物tRNA測(cè)序服務(wù)優(yōu)勢(shì)
● 特異性的tRNA測(cè)序文庫構(gòu)建方案，高tRNA檢出率及定量準(zhǔn)確性
● 優(yōu)化的tRNA去甲基化修飾流程，進(jìn)一步提升tRNA檢測(cè)分析效率
● 全面的tRNA轉(zhuǎn)錄組參考庫，綜合了所有的權(quán)威數(shù)據(jù)庫
● 詳盡的tRNA注釋信息與生物信息學(xué)分析
● 可直接用于文章發(fā)表的高質(zhì)量圖片
● 無縫鏈接康成生物提供的其它tRNA分析服務(wù)平臺(tái)，包括tRF&tiRNA-seq, tRF&tiRNA PCR array, tRNA Repertoire PCR array, tRNA Modification Enzymes PCR Array與LC-MS based tRNA Mod-ification analysis.

康成生物數(shù)據(jù)分析結(jié)果展示
1. 基本數(shù)據(jù)分析結(jié)果展示
1.1 tRNA表達(dá)定量
      康成生物利用Arraystar獨(dú)有的tRNA數(shù)據(jù)分析流程，提供信息全面的數(shù)據(jù)集（圖2）。除各tRNA表達(dá)量外，還額外提供有tRNA數(shù)據(jù)庫來源、基因組位置、堿基序列和二級(jí)結(jié)構(gòu)等相關(guān)信息，更好地為客戶后續(xù)科研助力圖2：全面的tRNA測(cè)序結(jié)果數(shù)據(jù)集

1.2 tRNA差異表達(dá)分析
      分析tRNA表達(dá)譜，挖掘差異表達(dá)tRNA分子；通過Heatmap、Scatter Diagram、Volcano Plot等統(tǒng)計(jì)圖形能夠清晰明確的展示樣本之間的tRNA表達(dá)差異性（圖3）。

圖3：tRNA差異表達(dá)Scatter Diagram和Volcano Plot示例。

參考文獻(xiàn)
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