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E.coli Expression

E.coli Expression


E.coli Expression
E.coli Expression
服務(wù)類別：其他生物研發(fā)總訪問：954
最后更新：2012-4-23半年訪問：21
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服務(wù)商： 北京百普賽斯生物科技有限公司 查看該公司所有服務(wù) >>
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E.coli used as host to make recombinant proteins for many years, and have advantages in terms of the speed,expense and productivity to get end products. The E.coli expression system is widely adopted as the primary choice for researchers and biotech companies to produce proteins for various applications..

ACRObiosystems utilizes the cost-effective solution for production of proteins that do not require glycosylation modifications using in-house developed expression vector and fermentation medium With ACRObiosystems' proprietary codon optimization tools, and highly effective expression and purification platform,greater than 5mg purified recombinant protein is guaranteed at cost of less than $1500.
* Risk-free trial for clients who just send DNA sequences via the request form, we'll take care of the rest.

Service Specification


Service Content Approaches  Deliverables Timeline

Gene synthesizing
&Palsmid construction
  • In-house developed codon optimization
  • Gene synthesizing and verification
  • Clone to proprietary expression vectors
  • Gene sequencing report
  • Constructed plasmid
 20 days
Fermentation
  • Seeds train expansion
  • 1- 50L E.coli Fermentation 
   2-4 days
Purification & QC
  • Sonication/clarification after harvest
  • Chromatography column purification
  • Analytical assay
  • Milligram to gram purified proteins
  • QC report
 3-5 days

Overlaped timeline: 2-3 weeks

Quotations and Ordering

Email:
Phone: +1 301-825-5518(US)   +44 20-8123-6518(EU)   +86 400-685-2052(AP)
Fax: +1 301-500-2214

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