反向LncRNA靶基因預(yù)測服務(wù)

服務(wù)內(nèi)容：
與常規(guī)的從lncRNA出發(fā)預(yù)測靶基因不同，反向long noncodingRNA(lncRNA)靶基因預(yù)測是從感興趣的已知基因開始（例如p53，COX-2等與腫瘤等密切相關(guān)的基因，這些基因可以來自芯片分析結(jié)果、NLP結(jié)果或客戶給定的任何基因列表），利用我們的longnoncoding RNA(lncRNA)數(shù)據(jù)庫和靶基因預(yù)測軟件pipeline，找到潛在的調(diào)節(jié)這些基因的lncRNA。

研究方法：
我們的服務(wù)提供：

1.獲取感興趣的基因集合

這些基因可以來自芯片分析結(jié)果、NLP結(jié)果或客戶給定的任何基因列表。如p53，COX-2。

2.建立lncRNA數(shù)據(jù)庫

我們通過整合已知所有l(wèi)ncRNA數(shù)據(jù)庫，形成一個全面的包含所有l(wèi)ncRNA序列的數(shù)據(jù)庫

3.細胞組織特異高表達lncRNA的鑒定（可選）

根據(jù)客戶提供的細胞組織信息，我們搜索已有的tiling芯片以及新一代測序得到的轉(zhuǎn)錄組信息，從全基因組已知lncRNA的中鑒定高表達的lncRNA。

4.cis靶基因預(yù)測

Cis作用[5]是lncRNA的一種靶基因作用方式。我們利用基因組注釋和基因組瀏覽器鑒定lncRNA的可能的靶基因。一般在啟動子區(qū)域同向轉(zhuǎn)錄的靶基因一般是促進表達作用，反向為抑制。而在3’-UTR區(qū)域時，部分情況下反向也為促進表達，見[14]。

5.trans靶基因預(yù)測

trans作用是lncRNA的另一種靶基因作用方式。我們利用RNAplex（http://www.tbi.univie.ac.at/~htafer/）等鑒定lncRNA的可能的靶基因，一個例子請見[15]。
綜上，一個可能分析流程如下：



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